First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America

Objectives: The emergence and spread of carbapenem resistant clones is of major concern for global health. This study aimed to characterize the first detected Klebsiella pneumoniae ST15 harboring the epi- demic carbapenemase OXA-48 in South America. Methods: During a routine colonization screening w...

সম্পূর্ণ বিবরণ

সংরক্ষণ করুন:
গ্রন্থ-পঞ্জীর বিবরন
প্রধান লেখক: Salazar González, María Cecilia (author)
অন্যান্য লেখক: Antelo Gutiérrez, Verónica Beatriz (author), Vieytes, Mariela (author), Dávila, Cristina (author), Grill, Fabio (author), Galiana, Antonio (author), Iraola, Gregorio (author)
বিন্যাস: article
ভাষা:ইংরেজি
প্রকাশিত: 2022
বিষয়গুলি:
অনলাইন ব্যবহার করুন:https://hdl.handle.net/20.500.12008/38461
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_version_ 1868889950455332864
author Salazar González, María Cecilia
author2 Antelo Gutiérrez, Verónica Beatriz
Vieytes, Mariela
Dávila, Cristina
Grill, Fabio
Galiana, Antonio
Iraola, Gregorio
author2_role author
author
author
author
author
author
author_browse Antelo Gutiérrez, Verónica Beatriz
Dávila, Cristina
Galiana, Antonio
Grill, Fabio
Iraola, Gregorio
Salazar González, María Cecilia
Vieytes, Mariela
author_facet Salazar González, María Cecilia
Antelo Gutiérrez, Verónica Beatriz
Vieytes, Mariela
Dávila, Cristina
Grill, Fabio
Galiana, Antonio
Iraola, Gregorio
author_role author
collection COLIBRI
dc.contributor.none.fl_str_mv Salazar González María Cecilia, Instituto Pasteur (Montevideo).
Antelo Gutiérrez Verónica Beatriz, Instituto Pasteur (Montevideo).
Vieytes Mariela
Dávila Cristina
Grill Fabio
Galiana Antonio
Iraola Gregorio, Instituto Pasteur (Montevideo).
dc.creator.none.fl_str_mv Salazar González, María Cecilia
Antelo Gutiérrez, Verónica Beatriz
Vieytes, Mariela
Dávila, Cristina
Grill, Fabio
Galiana, Antonio
Iraola, Gregorio
dc.date.none.fl_str_mv 2022
2023-07-27T13:01:58Z
2023-07-27T13:01:58Z
dc.format.none.fl_str_mv 5 h.
application/pdf
dc.identifier.none.fl_str_mv Salazar González, M, Antelo Gutiérrez, V, Vieytes, M, [y otros autores]. "First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America". Journal of Global Antimicrobial Resistance. [en línea] 2022, 30: 480-484. 5 h. DOI: 10.1016/j.jgar.2022.08.005
2213-7165
https://hdl.handle.net/20.500.12008/38461
10.1016/j.jgar.2022.08.005
dc.language.none.fl_str_mv en_US
eng
dc.publisher.none.fl_str_mv Elsevier Ltd
dc.relation.none.fl_str_mv Journal of Global Antimicrobial Resistance, 2022, 30: 480-484.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
dc.source.none.fl_str_mv reponame:COLIBRI
instname:Universidad de la República
instacron:Universidad de la República
dc.subject.none.fl_str_mv OXA-48
Carbapenem resistance
Klebsiella pneumonia
Multi-drug resistance
Whole-genome sequencing
dc.title.none.fl_str_mv First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America
dc.type.none.fl_str_mv Artículo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description Objectives: The emergence and spread of carbapenem resistant clones is of major concern for global health. This study aimed to characterize the first detected Klebsiella pneumoniae ST15 harboring the epi- demic carbapenemase OXA-48 in South America. Methods: During a routine colonization screening with carbapenem-resistant bacteria, one K. pneumoniae strain (CGHM01) was isolated from the urine of a hospitalized patient suffering from a neurodegenera- tive disease in Uruguay. We used long-read whole-genome sequencing and a phylogenomic approach to characterize the emergence of K. pneumoniae CGHM01. Results: K. pneumoniae CGHM01 is a multi-drug resistant strain carrying an IncL/M plasmid that encodes the carbapenemase gene bla OXA-48 within the Tn1999.2 transposon. Also, it carries an IncR plasmid har- boring a class I integron with an array of antibiotic resistance genes including the extended-spectrum beta-lactamase bla CTX-M-15 . Two copies of bla CTX-M-15 were also inserted in different positions of the chro- mosome. CGHM01 belongs to a ST15 sublineage that likely originated in continental Spain around 2012. Conclusions: The asymptomatic carriage of this strain in the urinary tract warns of difficulties for detec- tion and reporting of emerging carbapenem-resistant clones in new geographic areas where these are not endemic.
eu_rights_str_mv openAccess
format article
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identifier_str_mv Salazar González, M, Antelo Gutiérrez, V, Vieytes, M, [y otros autores]. "First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America". Journal of Global Antimicrobial Resistance. [en línea] 2022, 30: 480-484. 5 h. DOI: 10.1016/j.jgar.2022.08.005
2213-7165
10.1016/j.jgar.2022.08.005
instacron_str Universidad de la República
institution Universidad de la República
instname_str Universidad de la República
language eng
language_invalid_str_mv en_US
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oai_identifier_str oai:colibri.udelar.edu.uy:20.500.12008/38461
publishDate 2022
publishDateSort 2022
publisher.none.fl_str_mv Elsevier Ltd
reponame_str COLIBRI
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)
spelling First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South AmericaSalazar González, María CeciliaAntelo Gutiérrez, Verónica BeatrizVieytes, MarielaDávila, CristinaGrill, FabioGaliana, AntonioIraola, GregorioOXA-48Carbapenem resistanceKlebsiella pneumoniaMulti-drug resistanceWhole-genome sequencingObjectives: The emergence and spread of carbapenem resistant clones is of major concern for global health. This study aimed to characterize the first detected Klebsiella pneumoniae ST15 harboring the epi- demic carbapenemase OXA-48 in South America. Methods: During a routine colonization screening with carbapenem-resistant bacteria, one K. pneumoniae strain (CGHM01) was isolated from the urine of a hospitalized patient suffering from a neurodegenera- tive disease in Uruguay. We used long-read whole-genome sequencing and a phylogenomic approach to characterize the emergence of K. pneumoniae CGHM01. Results: K. pneumoniae CGHM01 is a multi-drug resistant strain carrying an IncL/M plasmid that encodes the carbapenemase gene bla OXA-48 within the Tn1999.2 transposon. Also, it carries an IncR plasmid har- boring a class I integron with an array of antibiotic resistance genes including the extended-spectrum beta-lactamase bla CTX-M-15 . Two copies of bla CTX-M-15 were also inserted in different positions of the chro- mosome. CGHM01 belongs to a ST15 sublineage that likely originated in continental Spain around 2012. Conclusions: The asymptomatic carriage of this strain in the urinary tract warns of difficulties for detec- tion and reporting of emerging carbapenem-resistant clones in new geographic areas where these are not endemic.Elsevier LtdSalazar González María Cecilia, Instituto Pasteur (Montevideo).Antelo Gutiérrez Verónica Beatriz, Instituto Pasteur (Montevideo).Vieytes MarielaDávila CristinaGrill FabioGaliana AntonioIraola Gregorio, Instituto Pasteur (Montevideo).2023-07-27T13:01:58Z2023-07-27T13:01:58Z2022Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion5 h.application/pdfSalazar González, M, Antelo Gutiérrez, V, Vieytes, M, [y otros autores]. "First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America". Journal of Global Antimicrobial Resistance. [en línea] 2022, 30: 480-484. 5 h. DOI: 10.1016/j.jgar.2022.08.0052213-7165https://hdl.handle.net/20.500.12008/3846110.1016/j.jgar.2022.08.005reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la Repúblicaen_USengJournal of Global Antimicrobial Resistance, 2022, 30: 480-484.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)oai:colibri.udelar.edu.uy:20.500.12008/384612026-04-14T10:10:09Z
spellingShingle First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America
Salazar González, María Cecilia
OXA-48
Carbapenem resistance
Klebsiella pneumonia
Multi-drug resistance
Whole-genome sequencing
status_str publishedVersion
title First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America
title_full First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America
title_fullStr First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America
title_full_unstemmed First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America
title_short First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America
title_sort First detection and origin of multi-drug resistant Klebsiella pneumoniae ST15 harboring OXA-48 in South America
topic OXA-48
Carbapenem resistance
Klebsiella pneumonia
Multi-drug resistance
Whole-genome sequencing
url https://hdl.handle.net/20.500.12008/38461