Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation
A variety of intestinal-derived culture systems have been developed to mimic in vivo cell behavior and organization, incorporating different tissue and microenvironmental elements. Great insight into the biology of the causative agent of toxoplasmosis, Toxoplasma gondii, has been attained by using d...
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| Other Authors: | , , , |
| Format: | article |
| Language: | English |
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2023
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| Online Access: | https://hdl.handle.net/20.500.12008/54033 |
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| _version_ | 1868889929263611904 |
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| author | Sena, Florencia |
| author2 | Cancela, Saira Bollati-Fogolín, Mariela Pagotto, Romina Francia, María E. |
| author2_role | author author author author |
| author_browse | Bollati-Fogolín, Mariela Cancela, Saira Francia, María E. Pagotto, Romina Sena, Florencia |
| author_facet | Sena, Florencia Cancela, Saira Bollati-Fogolín, Mariela Pagotto, Romina Francia, María E. |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Sena Florencia, Institut Pasteur Montevideo (Uruguay) Cancela Saira, Institut Pasteur Montevideo (Uruguay) Bollati-Fogolín Mariela, Institut Pasteur Montevideo (Uruguay) Pagotto Romina, Institut Pasteur Montevideo (Uruguay) Francia María E., Universidad de la República (Uruguay). Facultad de Medicina. Departamento de Parasitología y Micología |
| dc.creator.none.fl_str_mv | Sena, Florencia Cancela, Saira Bollati-Fogolín, Mariela Pagotto, Romina Francia, María E. |
| dc.date.none.fl_str_mv | 2023 2026-03-23T13:22:07Z 2026-03-23T13:22:07Z |
| dc.format.none.fl_str_mv | 15 p. application/pdf |
| dc.identifier.none.fl_str_mv | Sena F, Cancela S, Bollati-Fogolín M y otros. Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation. Frontiers in Cellular and Infection Microbiology [en línea]. 2023;13. 15 p. https://hdl.handle.net/20.500.12008/54033 10.3389/fcimb.2023.1134471 2235-2988 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | Frontiers Media |
| dc.relation.none.fl_str_mv | Frontiers in Cellular and Infection Microbiology. 2023;13 |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Toxoplasma gondii Ex vivo models Felinization In vitro models Intestine Sexual differentiation ANIMALES BIOLOGÍA GATOS RATONES MUCOSA INTESTINAL INTESTINOS TOXOPLASMA TOXOPLASMOSIS DIFERENCIACIÓN SEXUAL |
| dc.title.none.fl_str_mv | Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | A variety of intestinal-derived culture systems have been developed to mimic in vivo cell behavior and organization, incorporating different tissue and microenvironmental elements. Great insight into the biology of the causative agent of toxoplasmosis, Toxoplasma gondii, has been attained by using diverse in vitro cellular models. Nonetheless, there are still processes key to its transmission and persistence which remain to be elucidated, such as the mechanisms underlying its systemic dissemination and sexual differentiation both of which occur at the intestinal level. Because this event occurs in a complex and specific cellular environment (the intestine upon ingestion of infective forms, and the feline intestine, respectively), traditional reductionist in vitro cellular models fail to recreate conditions resembling in vivo physiology. The development of new biomaterials and the advances in cell culture knowledge have opened the door to a next generation of more physiologically relevant cellular models. Among them, organoids have become a valuable tool for unmasking the underlying mechanism involved in T. gondii sexual differentiation. Murine-derived intestinal organoids mimicking the biochemistry of the feline intestine have allowed the generation of pre-sexual and sexual stages of T. gondii for the first time in vitro, opening a window of opportunity to tackling these stages by "felinizing" a wide variety of animal cell cultures. Here, we reviewed intestinal in vitro and ex vivo models and discussed their strengths and limitations in the context of a quest for faithful models to in vitro emulate the biology of the enteric stages of T. gondii. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_0353db4f487004a45c01c41c0be3473a |
| identifier_str_mv | Sena F, Cancela S, Bollati-Fogolín M y otros. Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation. Frontiers in Cellular and Infection Microbiology [en línea]. 2023;13. 15 p. 10.3389/fcimb.2023.1134471 2235-2988 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/54033 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher.none.fl_str_mv | Frontiers Media |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiationSena, FlorenciaCancela, SairaBollati-Fogolín, MarielaPagotto, RominaFrancia, María E.Toxoplasma gondiiEx vivo modelsFelinizationIn vitro modelsIntestineSexual differentiationANIMALESBIOLOGÍAGATOSRATONESMUCOSA INTESTINALINTESTINOSTOXOPLASMATOXOPLASMOSISDIFERENCIACIÓN SEXUALA variety of intestinal-derived culture systems have been developed to mimic in vivo cell behavior and organization, incorporating different tissue and microenvironmental elements. Great insight into the biology of the causative agent of toxoplasmosis, Toxoplasma gondii, has been attained by using diverse in vitro cellular models. Nonetheless, there are still processes key to its transmission and persistence which remain to be elucidated, such as the mechanisms underlying its systemic dissemination and sexual differentiation both of which occur at the intestinal level. Because this event occurs in a complex and specific cellular environment (the intestine upon ingestion of infective forms, and the feline intestine, respectively), traditional reductionist in vitro cellular models fail to recreate conditions resembling in vivo physiology. The development of new biomaterials and the advances in cell culture knowledge have opened the door to a next generation of more physiologically relevant cellular models. Among them, organoids have become a valuable tool for unmasking the underlying mechanism involved in T. gondii sexual differentiation. Murine-derived intestinal organoids mimicking the biochemistry of the feline intestine have allowed the generation of pre-sexual and sexual stages of T. gondii for the first time in vitro, opening a window of opportunity to tackling these stages by "felinizing" a wide variety of animal cell cultures. Here, we reviewed intestinal in vitro and ex vivo models and discussed their strengths and limitations in the context of a quest for faithful models to in vitro emulate the biology of the enteric stages of T. gondii.Frontiers MediaSena Florencia, Institut Pasteur Montevideo (Uruguay)Cancela Saira, Institut Pasteur Montevideo (Uruguay)Bollati-Fogolín Mariela, Institut Pasteur Montevideo (Uruguay)Pagotto Romina, Institut Pasteur Montevideo (Uruguay)Francia María E., Universidad de la República (Uruguay). Facultad de Medicina. Departamento de Parasitología y Micología2026-03-23T13:22:07Z2026-03-23T13:22:07Z2023Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion15 p.application/pdfSena F, Cancela S, Bollati-Fogolín M y otros. Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation. Frontiers in Cellular and Infection Microbiology [en línea]. 2023;13. 15 p.https://hdl.handle.net/20.500.12008/5403310.3389/fcimb.2023.11344712235-2988reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengFrontiers in Cellular and Infection Microbiology. 2023;13Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/540332026-04-14T10:28:28Z |
| spellingShingle | Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation Sena, Florencia Toxoplasma gondii Ex vivo models Felinization In vitro models Intestine Sexual differentiation ANIMALES BIOLOGÍA GATOS RATONES MUCOSA INTESTINAL INTESTINOS TOXOPLASMA TOXOPLASMOSIS DIFERENCIACIÓN SEXUAL |
| status_str | publishedVersion |
| title | Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation |
| title_full | Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation |
| title_fullStr | Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation |
| title_full_unstemmed | Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation |
| title_short | Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation |
| title_sort | Exploring Toxoplasma gondii´s Biology within the Intestinal Epithelium: intestinal-derived models to unravel sexual differentiation |
| topic | Toxoplasma gondii Ex vivo models Felinization In vitro models Intestine Sexual differentiation ANIMALES BIOLOGÍA GATOS RATONES MUCOSA INTESTINAL INTESTINOS TOXOPLASMA TOXOPLASMOSIS DIFERENCIACIÓN SEXUAL |
| url | https://hdl.handle.net/20.500.12008/54033 |