Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis
Racemose neurocysticercosis is an aggressive disease caused by the aberrant expansión of the cyst form of Taenia solium within the subarachnoid spaces of the human brain and spinal cord resulting in a mass effect and chronic inflammation. Although expansion is likely caused by the proliferation and...
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| 格式: | article |
| 語言: | 英语 |
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2021
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| 在線閱讀: | https://hdl.handle.net/20.500.12008/37474 |
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| _version_ | 1868889923296165888 |
|---|---|
| author | Orrego, Miguel A |
| author2 | Verastegui, Manuela R Vasquez, Carlos M Koziol, Uriel Laclette, Juan P Garcia, Hector H Nash, Theodore E |
| author2_role | author author author author author author |
| author_browse | Garcia, Hector H Koziol, Uriel Laclette, Juan P Nash, Theodore E Orrego, Miguel A Vasquez, Carlos M Verastegui, Manuela R |
| author_facet | Orrego, Miguel A Verastegui, Manuela R Vasquez, Carlos M Koziol, Uriel Laclette, Juan P Garcia, Hector H Nash, Theodore E |
| author_role | author |
| collection | COLIBRI |
| dc.contributor.none.fl_str_mv | Orrego Miguel A Verastegui Manuela R Vasquez Carlos M Koziol Uriel, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Laclette Juan P Garcia Hector H Nash Theodore E |
| dc.creator.none.fl_str_mv | Orrego, Miguel A Verastegui, Manuela R Vasquez, Carlos M Koziol, Uriel Laclette, Juan P Garcia, Hector H Nash, Theodore E |
| dc.date.none.fl_str_mv | 2021 2023-06-07T16:12:54Z 2023-06-07T16:12:54Z |
| dc.format.none.fl_str_mv | 18 h application/pdf |
| dc.identifier.none.fl_str_mv | Orrego, M, Verastegui, M, Vasquez, C [y otros autores] "Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis". PLOS Neglected Tropical Diseases. [en línea] 2021, 15(3): e0009303. 18 h. DOI: 10.1371/journal.pntd.0009303. https://hdl.handle.net/20.500.12008/37474 10.1371/journal.pntd.0009303 1935-2735 |
| dc.language.none.fl_str_mv | en eng |
| dc.publisher.none.fl_str_mv | PLOS |
| dc.relation.none.fl_str_mv | PLOS Neglected Tropical Diseases, 2021, 15(3): e0009303. |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.none.fl_str_mv | Racemose neurocysticercosis Taenia solium Serine/threonine-protein kinase plk1 |
| dc.title.none.fl_str_mv | Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis |
| dc.type.none.fl_str_mv | Artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| description | Racemose neurocysticercosis is an aggressive disease caused by the aberrant expansión of the cyst form of Taenia solium within the subarachnoid spaces of the human brain and spinal cord resulting in a mass effect and chronic inflammation. Although expansion is likely caused by the proliferation and growth of the parasite bladder wall, there is little direct evidence of the mechanisms that underlie these processes. Since the development and growth of cysts in related cestodes involves totipotential germinative cells, we hypothesized that the expansive growth of the racemose larvae is organized and maintained by germinative cells. Here, we identified proliferative cells expressing the serine/threonine-protein kinase plk1 by in situ hybridization. Proliferative cells were present within the bladder wall of racemose form and absent from the homologous tissue surrounding the vesicular form. Cyst proliferation in the related model species Taenia crassiceps (ORF strain) occurs normally by budding from the cyst bladder wall and proliferative cells were concentrated within the growth buds. Cells isolated from bladder wall of racemose larvae were established in primary cell culture and insulin stimulated their proliferation in a dose-dependent manner. These findings indicate that the growth of racemose larvae is likely due to abnormal cell proliferation. The different distribution of proliferative cells in the racemose larvae and their sensitivity to insulin may reflect significant changes at the cellular and molecular levels involved in their tumor-like growth. Parasite cell cultures offer a powerful tool to characterize the nature and formation of the racemose form, understand the developmental biology of T. solium, and to identify new effective drugs for treatment. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | anni_01ac81c2933b29e180e34d5beb56a4c1 |
| identifier_str_mv | Orrego, M, Verastegui, M, Vasquez, C [y otros autores] "Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis". PLOS Neglected Tropical Diseases. [en línea] 2021, 15(3): e0009303. 18 h. DOI: 10.1371/journal.pntd.0009303. 10.1371/journal.pntd.0009303 1935-2735 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | anni |
| network_name_str | oai-lr-anni |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/37474 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| publisher.none.fl_str_mv | PLOS |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosisOrrego, Miguel AVerastegui, Manuela RVasquez, Carlos MKoziol, UrielLaclette, Juan PGarcia, Hector HNash, Theodore ERacemose neurocysticercosisTaenia soliumSerine/threonine-protein kinase plk1Racemose neurocysticercosis is an aggressive disease caused by the aberrant expansión of the cyst form of Taenia solium within the subarachnoid spaces of the human brain and spinal cord resulting in a mass effect and chronic inflammation. Although expansion is likely caused by the proliferation and growth of the parasite bladder wall, there is little direct evidence of the mechanisms that underlie these processes. Since the development and growth of cysts in related cestodes involves totipotential germinative cells, we hypothesized that the expansive growth of the racemose larvae is organized and maintained by germinative cells. Here, we identified proliferative cells expressing the serine/threonine-protein kinase plk1 by in situ hybridization. Proliferative cells were present within the bladder wall of racemose form and absent from the homologous tissue surrounding the vesicular form. Cyst proliferation in the related model species Taenia crassiceps (ORF strain) occurs normally by budding from the cyst bladder wall and proliferative cells were concentrated within the growth buds. Cells isolated from bladder wall of racemose larvae were established in primary cell culture and insulin stimulated their proliferation in a dose-dependent manner. These findings indicate that the growth of racemose larvae is likely due to abnormal cell proliferation. The different distribution of proliferative cells in the racemose larvae and their sensitivity to insulin may reflect significant changes at the cellular and molecular levels involved in their tumor-like growth. Parasite cell cultures offer a powerful tool to characterize the nature and formation of the racemose form, understand the developmental biology of T. solium, and to identify new effective drugs for treatment.PLOSOrrego Miguel AVerastegui Manuela RVasquez Carlos MKoziol Uriel, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Laclette Juan PGarcia Hector HNash Theodore E2023-06-07T16:12:54Z2023-06-07T16:12:54Z2021Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion18 happlication/pdfOrrego, M, Verastegui, M, Vasquez, C [y otros autores] "Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis". PLOS Neglected Tropical Diseases. [en línea] 2021, 15(3): e0009303. 18 h. DOI: 10.1371/journal.pntd.0009303.https://hdl.handle.net/20.500.12008/3747410.1371/journal.pntd.00093031935-2735reponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengPLOS Neglected Tropical Diseases, 2021, 15(3): e0009303.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/374742026-04-14T10:10:06Z |
| spellingShingle | Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis Orrego, Miguel A Racemose neurocysticercosis Taenia solium Serine/threonine-protein kinase plk1 |
| status_str | publishedVersion |
| title | Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis |
| title_full | Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis |
| title_fullStr | Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis |
| title_full_unstemmed | Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis |
| title_short | Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis |
| title_sort | Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis |
| topic | Racemose neurocysticercosis Taenia solium Serine/threonine-protein kinase plk1 |
| url | https://hdl.handle.net/20.500.12008/37474 |