Genome-wide chromatin interaction map for Trypanosoma cruzi

Trypanosomes are eukaryotic, unicellular parasites, such as Trypanosoma brucei, which causes sleeping sickness, and Trypanosoma cruzi, which causes Chagas disease. Genomes of these parasites comprise core regions and species-specific disruptive regions that encode multigene families of surface glyco...

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Egile nagusia: Díaz-Viraqué, Florencia (author)
Beste egile batzuk: Chiribao, María Laura (author), Libisch, María Gabriela (author), Robello, Carlos (author)
Formatua: article
Hizkuntza:ingelesa
Argitaratua: 2023
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Sarrera elektronikoa:https://hdl.handle.net/20.500.12008/52593
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author Díaz-Viraqué, Florencia
author2 Chiribao, María Laura
Libisch, María Gabriela
Robello, Carlos
author2_role author
author
author
author_browse Chiribao, María Laura
Díaz-Viraqué, Florencia
Libisch, María Gabriela
Robello, Carlos
author_facet Díaz-Viraqué, Florencia
Chiribao, María Laura
Libisch, María Gabriela
Robello, Carlos
author_role author
collection COLIBRI
dc.contributor.none.fl_str_mv Díaz-Viraqué Florencia, Institut Pasteur de Montevideo (Uruguay)
Chiribao María Laura, Universidad de la República (Uruguay). Facultad de Medicina
Libisch María Gabriela, Institut Pasteur de Montevideo (Uruguay)
Robello Carlos, Universidad de la República (Uruguay). Facultad de Medicina
dc.creator.none.fl_str_mv Díaz-Viraqué, Florencia
Chiribao, María Laura
Libisch, María Gabriela
Robello, Carlos
dc.date.none.fl_str_mv 2023
2025-11-21T17:47:23Z
2025-11-21T17:47:23Z
dc.format.none.fl_str_mv 19 p.
application/pdf
dc.identifier.none.fl_str_mv Díaz-Viraqué F, Chiribao M, Libisch M y otros. Genome-wide chromatin interaction map for Trypanosoma cruzi. Nature Microbiology [en línea]. 2023;8 (11):2103-2114
2058-5276
https://hdl.handle.net/20.500.12008/52593
10.1038/s41564-023-01483-y
dc.language.none.fl_str_mv en
eng
dc.publisher.none.fl_str_mv Springer Nature
dc.relation.none.fl_str_mv Nature Microbiology. 2023;8 (11):2103-2114
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Licencia Creative Commons Atribución (CC - By 4.0)
dc.source.none.fl_str_mv reponame:COLIBRI
instname:Universidad de la República
instacron:Universidad de la República
dc.subject.none.fl_str_mv CROMATINA
CROMOSOMAS
GENÉTICA
GLICOPROTEÍNAS DE MEMBRANA
TRYPANOSOMA BRUCEI BRUCEI
TRYPANOSOMA CRUZI
dc.title.none.fl_str_mv Genome-wide chromatin interaction map for Trypanosoma cruzi
dc.type.none.fl_str_mv Artículo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
description Trypanosomes are eukaryotic, unicellular parasites, such as Trypanosoma brucei, which causes sleeping sickness, and Trypanosoma cruzi, which causes Chagas disease. Genomes of these parasites comprise core regions and species-specific disruptive regions that encode multigene families of surface glycoproteins. Few transcriptional regulators have been identified in these parasites, and the role of spatial organization of the genome in gene expression is unclear. Here we mapped genome-wide chromatin interactions in T. cruzi using chromosome conformation capture (Hi-C), and we show that the core and disruptive regions form three-dimensional chromatin compartments named C and D. These chromatin compartments differ in levels of DNA methylation, nucleosome positioning and chromatin interactions, affecting genome expression dynamics. Our data reveal that the trypanosome genome is organized into chromatin-folding domains and transcription is affected by the local chromatin structure. We propose a model in which epigenetic mechanisms affect gene expression in trypanosomes.
eu_rights_str_mv openAccess
format article
id anni_016fa4b8bfb708c3cb1a6997fdb3d9fe
identifier_str_mv Díaz-Viraqué F, Chiribao M, Libisch M y otros. Genome-wide chromatin interaction map for Trypanosoma cruzi. Nature Microbiology [en línea]. 2023;8 (11):2103-2114
2058-5276
10.1038/s41564-023-01483-y
instacron_str Universidad de la República
institution Universidad de la República
instname_str Universidad de la República
language eng
language_invalid_str_mv en
network_acronym_str anni
network_name_str oai-lr-anni
oai_identifier_str oai:colibri.udelar.edu.uy:20.500.12008/52593
publishDate 2023
publishDateSort 2023
publisher.none.fl_str_mv Springer Nature
reponame_str COLIBRI
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv Licencia Creative Commons Atribución (CC - By 4.0)
spelling Genome-wide chromatin interaction map for Trypanosoma cruziDíaz-Viraqué, FlorenciaChiribao, María LauraLibisch, María GabrielaRobello, CarlosCROMATINACROMOSOMASGENÉTICAGLICOPROTEÍNAS DE MEMBRANATRYPANOSOMA BRUCEI BRUCEITRYPANOSOMA CRUZITrypanosomes are eukaryotic, unicellular parasites, such as Trypanosoma brucei, which causes sleeping sickness, and Trypanosoma cruzi, which causes Chagas disease. Genomes of these parasites comprise core regions and species-specific disruptive regions that encode multigene families of surface glycoproteins. Few transcriptional regulators have been identified in these parasites, and the role of spatial organization of the genome in gene expression is unclear. Here we mapped genome-wide chromatin interactions in T. cruzi using chromosome conformation capture (Hi-C), and we show that the core and disruptive regions form three-dimensional chromatin compartments named C and D. These chromatin compartments differ in levels of DNA methylation, nucleosome positioning and chromatin interactions, affecting genome expression dynamics. Our data reveal that the trypanosome genome is organized into chromatin-folding domains and transcription is affected by the local chromatin structure. We propose a model in which epigenetic mechanisms affect gene expression in trypanosomes.Springer NatureDíaz-Viraqué Florencia, Institut Pasteur de Montevideo (Uruguay)Chiribao María Laura, Universidad de la República (Uruguay). Facultad de MedicinaLibisch María Gabriela, Institut Pasteur de Montevideo (Uruguay)Robello Carlos, Universidad de la República (Uruguay). Facultad de Medicina2025-11-21T17:47:23Z2025-11-21T17:47:23Z2023Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion19 p.application/pdfDíaz-Viraqué F, Chiribao M, Libisch M y otros. Genome-wide chromatin interaction map for Trypanosoma cruzi. Nature Microbiology [en línea]. 2023;8 (11):2103-21142058-5276https://hdl.handle.net/20.500.12008/5259310.1038/s41564-023-01483-yreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaenengNature Microbiology. 2023;8 (11):2103-2114Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)oai:colibri.udelar.edu.uy:20.500.12008/525932026-04-14T10:28:22Z
spellingShingle Genome-wide chromatin interaction map for Trypanosoma cruzi
Díaz-Viraqué, Florencia
CROMATINA
CROMOSOMAS
GENÉTICA
GLICOPROTEÍNAS DE MEMBRANA
TRYPANOSOMA BRUCEI BRUCEI
TRYPANOSOMA CRUZI
status_str publishedVersion
title Genome-wide chromatin interaction map for Trypanosoma cruzi
title_full Genome-wide chromatin interaction map for Trypanosoma cruzi
title_fullStr Genome-wide chromatin interaction map for Trypanosoma cruzi
title_full_unstemmed Genome-wide chromatin interaction map for Trypanosoma cruzi
title_short Genome-wide chromatin interaction map for Trypanosoma cruzi
title_sort Genome-wide chromatin interaction map for Trypanosoma cruzi
topic CROMATINA
CROMOSOMAS
GENÉTICA
GLICOPROTEÍNAS DE MEMBRANA
TRYPANOSOMA BRUCEI BRUCEI
TRYPANOSOMA CRUZI
url https://hdl.handle.net/20.500.12008/52593